Our rapid assay to monitor phagocytosis can be used to study neutrophil deficiencies and bacterial evasion, but also provides a powerful tool to assess the opsonic capacity of antibodies, either in the context of natural immune responses or immune therapies.

2020年6月19日 · To study how phagocytosis is affected by specific changes to the target surface, we developed an in vitro assay based on reconstituted membrane-coated target particles to which known molecules...

2015年1月1日 · We shall now detail two phagocytosis assays that measure the uptake of either mammalian (host) cells, quantified by fluorescent microscopy or an FACS-based assay for determining the extent of phagocytosis of mycobacteria.

Phagocytosis of invading pathogens by macrophages represents a fundamental component of the innate immune system. In this chapter, we describe protocols designed for high-throughput analysis of phagosome formation and maturation using latex beads as model phagocytic targets.

2018年5月15日 · This chapter describes methods to induce and quantify phagocytosis in primary macrophages and in myeloid cell lines. To this end, we initially detail the isolation of primary human monocytes and their differentiation into macrophages.

We comprehensively evaluated and optimized phagocytosis assays using particles labeled with fluorescent pH-sensitive pHrodo ® dyes, facilitating the specific detection of phagocytosed particles.

3 天之前 · Phagocytosis assay. Effect of drug treatment on phagocytosis was performed using the Vybrant Phagocytosis Assay (Thermo Fisher) according to manufacturer’s instructions. THP-1 cells were cultured in 6-well plates at a seeding density of 1.5 x 10 6 cells/well and grown to confluence. Cells were treated with metformin or ZLN005 for 24 hours ...

2020年2月21日 · We comprehensively evaluated and optimized phagocytosis assays using particles labeled with fluorescent pH-sensitive pHrodo ® dyes, facilitating the specific detection of phagocytosed particles.

This chapter describes methods to induce and quantify phagocytosis in primary macrophages and in myeloid cell lines. To this end, we initially detail the isolation of primary human monocytes and their differentiation into macrophages.

2021年1月1日 · We use reference human phagocyte THP-1 cells with different prey and opsonization conditions to compare the persistent association-based normalization method to standard ways of assessing phagocytosis and find it to perform better, exhibiting increased robustness, sensitivity, and reproducibility.