Next-generation sequencing (NGS) is a modern method of analyzing genetic material that allows for the rapid sequencing of large amounts of DNA or RNA. Unlike traditional sequencing techniques, NGS can simultaneously sequence millions of small fragments of DNA, enabling researchers to expand the scale and discovery power of their genomic studies ...

Next-generation sequencing (NGS) is a massively parallel sequencing technology that offers ultra-high throughput, scalability, and speed. The technology is used to determine the order of nucleotides in entire genomes or targeted regions of DNA or RNA.

Next-generation sequencing workflows start with nucleic acid isolation, followed by library preparation. Libraries are sequenced on Illumina sequencing systems, designed to support a wide range of applications and throughputs.

Mitochondrial sequencing with next-generation sequencing (NGS) technology addresses these challenges, enabling comprehensive detection and analysis of mitochondrial disease-associated variants. NGS also enables mitochondrial DNA analysis for other applications such as human identification, forensics, and cancer research.

These next-generation sequencing (NGS) tutorials are designed to help you understand key concepts in NGS. With videos, online training, and technical bulletins, we’ll guide you through tips and best practices for library prep, sequencing, and data analysis.

Use our next-generation sequencing glossary to view definitions of key terms and clarify important concepts as you plan your sequencing project. The critical difference between Sanger sequencing and NGS is sequencing volume.

In principle, the concepts behind Sanger vs next-generation sequencing (NGS) technologies are similar. In both NGS and Sanger sequencing (also known as dideoxy or capillary electrophoresis sequencing), DNA polymerase adds fluorescent nucleotides one by one onto a growing DNA template strand.

Illumina DNA Prep, previously known as Nextera DNA Flex, offers a fast, robust, and flexible workflow for preparing normalized, sequencing-ready libraries from a wide range of DNA input types and amounts facilitating an array of applications, from human whole-genome sequencing to sequencing amplicons, plasmids, and microbial species. 1

Using NGS in key research areas. NGS can play an important role in pursuing the answer to a variety of biological questions using a wide array of published methods for diverse sample types. NGS enables the unbiased investigation of multiple biological “omes”, such as the proteome, transcriptome, epigenome, and genome.

Illumina sequencing enables a wide variety of applications, allowing researchers to ask virtually any question related to the genome, transcriptome, or epigenome of any organism. Next-generation sequencing (NGS) methods differ primarily by how the DNA or RNA samples are prepared and the data analysis options used.