These conventional immunofluorescence studies were bolstered by elegant 3D correlative light and electron microscopy studies (“CLEM”) that indicated a loss of approximately one-third the volume of mitotic chromosomes upon depletion of Ki-67 (Booth et al. 2016).

Confocal immunofluorescence images show Ki67+ nuclei (red) in heart sections from mice and total nuclei stained with DAPI (blue). Overlaid image shows overlap (arrowheads) of Ki67+ nuclei with DAPI. Scale bar = 50 μm.

Ki67 staining is widely used as a proliferation indicator in the clinic, despite poor understanding of this protein’s function or dynamics. Here, we track Ki67 levels under endogenous control in single cells over time and find that Ki67 accumulation occurs only during S, G2, and M phases.

2014年1月22日 · In this study, we established a reliable protocol for Ki-67 staining in murine cells and tissue based on PFA fixation, which can be used not only for flow cytometry but also for immunofluorescence microscopy analysis. We tested our protocol successfully with three different Ki-67 anti-mouse antibodies in cell culture, regenerating liver tissue ...

In this structure, Ki-67 acts to prevent aggregation of mitotic chromosomes. Here, we present an overview of functional roles of Ki-67 across the cell cycle and also describe recent experiments that clarify its role in regulating cell cycle progression in human cells.

In this chapter, we illustrate how to perform immunofluorescence to detect the localization of Ki-67 (a marker of actively growing/proliferating chondrocytes) in order to assess the growth fraction of the columnar chondrocytes in the growth plate in paraffin-embedded mouse tissue limb.

2018年10月30日 · Purpose: This protocol describes how to stain sections of formalin-fixed paraffin-embedded tissue for Ki67, a protein marker of proliferation expressed during S, G2, and M phase of the cell cycle.

This protocol describes how to stain sections of formalin-fixed paraffin-embedded tissue for Ki67, a protein marker of proliferation expressed during S, G2, and M phase of the cell cycle.

2013年12月8日 · Prognostic and predictive value of centrally reviewed Ki-67 labeling index in postmenopausal women with endocrine-responsive breast cancer: results from Breast International Group Trial 1–98 comparing adjuvant tamoxifen with letrozole.

Research of Ki-67 expression measured using quantitative immunofluorescence automated quantitative analysis (AQUA) technology found that high Ki-67 levels are a predictor of neoadjuvant chemotherapy response .