Thermolysin (EC 3.4.24.27, Bacillus thermoproteolyticus neutral proteinase, thermoase, thermoase Y10, TLN) is a thermostable neutral metalloproteinase enzyme produced by the Gram-positive bacteria Bacillus thermoproteolyticus. [2] It requires one zinc ion for enzyme activity and four calcium ions for structural stability. [3]

Thermolysin from Bacillus thermoproteolyticus was the first metalloendopeptidase whose 3D structure was solved by X-ray crystallography (Matthews et al. 1972, 1974; Colman et al. 1972). Thermolysin consists of a two-domain structure with the active site between the domains.

2008年12月18日 · The thermolysin family of enzymes is classified as the M4 family of metallopeptidases (http://merops.sanger.ac.uk/). At least 200 (in 2007) sequences belong to the M4 family, and they are found in bacteria, fungi, and archaea. Thermolysin from Bacillus thermoproteolyticus is the type-example of the M4 family enzymes. All peptidases in the ...

Thermolysin is an extracellular metalloendopeptidase from the thermophile Bacillus thermoproteolyticus that has an optimal pH range of 7.0–9.0 and an optimal reaction temperature of 70 °C. From: Comprehensive Sampling and Sample Preparation, 2012

Thermolysin is the name first given to an extracellular 34.6 kDa metalloendopeptidase secreted by the grampositive thermophilic bacterium Bacillus thermoproteolyticus (Endo, 1962). It was the first metalloendopeptidase to be crystallized and to have its structure solved (reviewed in Matthews, 1988). There is some confusion over the naming of ...

2004年1月1日 · Thermolysin is the name first given to an extracellular 34.6 kDa metalloendopeptidase secreted by the grampositive thermophilic bacterium Bacillus thermoproteolyticus (Endo, 1962). It was the first metalloendopeptidase to be crystallized and to have its structure solved (reviewed in Matthews, 1988). …

嗜热菌蛋白酶是一种生物学上的物质，外文名是Thermolysin。特性是水解疏水性强的氨基酸时速度较快。

Thermolysin is an extracellular proteolytic enzyme isolated from Bacillus thermoproteolyticus (Endo, 1962). The enzyme is quite thermostable, retaining over half of its activity after being heated in an aqueous solution for an hour at 80°C, while at 65°C...

Some enzymes of the family are able to function at the extremes of temperatures, and some function in organic solvents. Thereby enzymes of the thermolysin family have an innovative potential for biotechnological applications.

2020年2月19日 · In native thermolysin, Zn 2+ at the active site is tetrahedrally coordinated by His142, His146, Glu166, and water molecule and contains three Ca 2+ ions, which are involved in thermostability. In the crystal structure of 1,10-phenanthroline-treated thermolysin crystal, seven 1,10-phenanthroline molecules were observed on the surface of thermolysin.