Immunofluorescence (IF) is a light microscopy-based technique that allows detection and localization of a wide variety of target biomolecules within a cell or tissue at a quantitative level. The technique utilizes the binding specificity of antibodies and antigens. [1]

2022年3月1日 · Immunofluorescence is a type of assay performed on biological samples to detect specific antigens in any biological specimen or sample and vice-versa. The specificity of antibodies to their antigen is the base for immunofluorescence.

Immunofluorescence (IF) is a technique that permits visualization of virtually many components in any given tissue or cell type. This broad capability is achieved through combinations of specific antibodies tagged with fluorophores.

Immunofluorescence assay is based on specific antibodies for detecting and visualizing particular proteins or antigens in biological samples using fluorescence microscopy. The immunofluorescence assay principle relies on the antibodies’ specificity for their target proteins.

2024年4月9日 · Immunofluorescence is a laboratory technique used to detect specific proteins or antigens in biological samples by using antibodies labeled with fluorescent molecules, allowing visualization under a fluorescence microscope.

2024年7月27日 · Explore the principles, types, and applications of modern immunofluorescence techniques, including sample preparation and quantitative analysis methods. In recent years, immunofluorescence has emerged as a pivotal tool in biological and medical research.

2023年9月1日 · Immunofluorescence assay (IFA) is a laboratory technique used for detecting and visualizing specific antigens or antibodies in biological samples. It combines the principles of both immunology and fluorescence microscopy to provide highly specific and sensitive results.

2021年11月16日 · What is immunofluorescence? Generally speaking, IF is an application in which antibodies that have been joined to fluorescent molecules are applied to a cell or tissue sample where they bind to their targets, most commonly proteins.

Immunofluorescence (FA) is used to detect viral antigens in clinical specimens (Fig. 10.42) or to confirm the presence of HSV in cell cultures inoculated with clinical materials. Acceptable specimens for FA diagnosis include lesion scrapings or vesicular fluid.

2020年12月16日 · What is immunofluorescence? Immunofluorescence is one of the techniques used within light microscopy, especially on microbiological samples. It uses a fluorescence microscope to observe antibodies, bonded to their antigens, with fluorescent dyes attached to specific biomolecule targets.