Epigenetic engineering of yeast reveals dynamic molecular …
2020年3月18日 · By introducing de novo DNA methyltransferases in a simple eukaryote that naturally lacks DNA methylation, we have characterized molecular adaptation of cells to ectopic DNA methylation stress, genetic information that guides the deposition of this epigenetic mark, …
Widespread existence of cytosine methylation in yeast DNA
2012年8月21日 · In the current study, we developed a highly sensitive method based on gas chromatography/mass spectrometry (GC/MS) and systematically examined the incidence of …
Widespread Existence of Cytosine Methylation in Yeast DNA …
Our results showed that DNA methylation is widespread in yeast and the genome-wide DNA methylation of the studied yeast strains ranged from 0.014 to 0.364%, which were 1 to 2 …
Impact of DNA methylation on 3D genome structure
2021年5月28日 · We studied a simpler model system, budding yeast, that lacks DNA methylation machinery making it a perfect model system to study the intrinsic role of DNA methylation in …
DNA methylation-based high-resolution mapping of long …
2024年5月22日 · The MTAC method is based on methylation of DNA physically proximal to a target site. A GpC methyltransferase, M.CviPI, is fused with LacI and recruited to the VP …
Genome-wide Map of Nucleosome Acetylation and Methylation in …
2005年8月26日 · We profiled nucleosome modifications across the yeast genome using chromatin immunoprecipitation coupled with DNA microarrays to produce high-resolution genome-wide …
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Evolutionary Persistence of DNA Methylation for Millions of Years …
2020年1月23日 · In the yeast Cryptococcus neoformans, CG methylation occurs in transposon-rich repeats and requires the DNA methyltransferase Dnmt5. We show that Dnmt5 displays …
Understanding the relationship between DNA methylation and …
2014年12月1日 · Interestingly, the antagonistic effects of H3K4me3 on DNA methylation are phenocopied when Dnmt3a is ectopically expressed in budding yeast, a fungus that lacks …
In vivo targeting of de novo DNA methylation by histone
2015年4月7日 · Our data demonstrate that DNMT3B and H3K4 methylation are mutually exclusive and that DNMT3B is co-localized with H3K36 methylated regions. In support of this …
Different levels of DNA methylation in yeast and mycelial forms of ...
Isotope dilution gas chromatography-mass spectrometry analysis of genomic DNAs isolated from three Candida albicans isolates showed significant differences in the amounts of 5 …
