2024年3月21日 · We then developed an evolution-inspired “eXchange Unit between T domains” (XUT) approach, which allows the assembly of NRPS fragments over a broad range of GC contents, protein similarities, and extender unit specificities, as demonstrated for the specific production of a proteasome inhibitor designed and assembled from five different NRPS ...

2024年3月22日 · In this work, we describe a detailed phylogenetic analysis of several bacterial NRPSs that led to the identification of yet undescribed recombination sites within the thiolation (T) domain that can be used for NRPS engineering.

2022年12月2日 · Here we describe a detailed phylogenetic analysis of several bacterial NRPS that led to the identification of a new recombination breakpoint within the thiolation (T) domain important in natural NRPS evolution.

2022年12月8日 · Here we describe a detailed phylogenetic analysis of several bacterial NRPS that led to the identification of a new recombination breakpoint within the thiolation (T) domain that is important for natural NRPS evolution.

2023年5月16日 · From this an evolutionary-inspired eXchange Unit between T domains (XUT) approach was developed, which allows the assembly of NRPS fragments over a broad range of GC contents, protein...

Our work describes the engineering of a designed NRPS/PKS hybrid via the assembly of different megasynthetase fragments to produce a rationally modified syrbactin derivative with a complex peptide-polyketide structure, acting as proteasome inhibitor.

2022年12月8日 · Since NRPS are not limited to the incorporation of the 20 proteinogenic amino. analogues, e.g. to optimise natural product leads. Here we describe a detailed. NRPS evolution. From this, an evolution-inspired eXchange Unit between T domains. assembled from five different NRPS fragments.

2022年12月2日 · Here we describe a detailed phylogenetic analysis of several bacterial NRPS that led to the identification of a new recombination breakpoint within the thiolation (T) domain important in natural...

2022年12月8日 · An evolution-inspired eXchange Unit between T domains (XUT) approach was developed which allows the assembly of NRPS fragments over a broad range of GC contents, protein similarities, and extender unit specificities, as demonstrated for the specific production of a proteasome inhibitor designed and assembled from five differentNRPS fragments.

2024年10月10日 · Here, we describe the stepwise reassembly of an entire NRPS/PKS hybrid to produce a new syrbactin derivative by utilizing the recently described “eXchange Unit between Thiolation domains” (XUTs) approach.