All oligonucleotides are made by phosphoramidite solid-phase synthesis. The steps in solid phase synthesis, and the preparation of reagents.

In contrast to organic solid-phase synthesis and peptide synthesis, the synthesis of oligonucleotides proceeds best on non-swellable or low-swellable solid supports. The two most often used solid-phase materials are controlled pore …

Methods for solid-phase synthesis of oligonucleotides. (A) Column-based oligonucleotide synthesis. This is the traditional method of synthesizing DNA using solid-phase phosphoramidite chemistry. The synthesis of a unique oligonucleotide sequence is done on the surface of controlled-porosity glass beads (CPG) contained with a synthesis column.

Avery’s realization that DNA carries the genetic information lead chemists on a 40-year search that has culminated in efficient, automated oligonucleotide synthesis on solid-phase supports. Modern nucleic acid synthesizers utilize phosphite triester chemistries that employ stable phosphoramidite monomers to build the growing polymer (see UNIT 3.3).

Phosphoramidite chemistry, developed in the 1980s and later enhanced with solid-phase supports and automation, is the method of choice for DNA oligonucleotide manufacturing. Opposite of biosynthesis, chemical synthesis proceeds in the 3' → 5' …

use in laboratories around the world. For most applications, very small quantities of DNA are required, and oligonucleotide synthesis is performed. mainly on the 40 nmol scale or lower. This provides ample quantities for most b.

In this review, the main focus is on supports for oligodeoxyribonucleotide and oligoribonu-cleotide synthesis. The synthetic strategies are often similar, particularly when synthetic li-braries are prepared.

Solid phase oligonucleotide synthesis of DNA is an established and reliable method for the synthesis of specific-sequence short-strand, up to ∼150-mer, DNA. As outlined in the introduction, many exciting therapeutic and diagnostic avenues are being explored using synthetic RNA, further highlighting the need for dependable and efficient ...

2023年3月1日 · Solid phase oligonucleotide synthesis uses a robust synthesis cycle to build up an oligonucleotide one base at a time, providing complete control over the oligonucleotide sequence (Fig. 1). Synthesis starts with deprotection of the 5′-hydroxyl group of the nucleoside attached to the solid support.

A commercially available PEG-PS support functionalized with either hydroxyl or amino groups has been used for solid-phase oligonucleotide synthesis using H-phosphonate-coupling chemistry . This support was advantageous in large (30 µmol) scale synthesis, because a high nucleoside loading (170 µmol/g) that was four- to fivefold greater than ...