The Raman microscope begins with a standard optical microscope, and adds an excitation laser, laser rejection filters, a spectrometer or monochromator, and an optical sensitive detector such as a charge-coupled device (CCD), or photomultiplier tube, (PMT).

显微拉曼是传统光学显微镜和拉曼光谱仪独特的化学鉴别优势的有机组合体。 这两种分析技术本身都相当强大，但将他们结合在一起之后，则可能对最小物体（> 0.5 μm）进行化学分析，因此，这两种技术的结合将光谱与空间信息联系了起来。 与显微红外相比，显微拉曼更容易信号过饱和，因为显微拉曼使用的光需要与简单的玻璃光学器件兼容。 因此，高质量的光学显微镜是显微拉曼研发的基础。 关于采样和共聚焦. 通常，根据分析任务，显微拉曼不需要繁杂的样本预处理。 通常 …

Raman microscopy (µ-Raman) combines the chemical analysis technique, Raman spectroscopy, with a traditional light microscope. This combination provides a very streamlined, “point and shoot” approach to Raman spectroscopy of very tiny objects.

Essentially, Raman microscopy is conducted by integrating an optical microscope to a Raman spectrometer with a laser for excitation. A Renishaw spectrometer/imaging microscope equipped with an X–Y micro-translating stage has been successfully used in surface and cross-section chemical line profiling and area mapping.

2024年12月11日 · Raman microscopy is an emerging tool for molecular analysis of biological specimens under physiological conditions (1, 2). Distributions of biomolecules in a sample are imaged by obtaining the spatial distributions of Raman scattering, allowing the visualization of cell dynamics (3), drug effects (4 – 6), and cell differentiations (7, 8).

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions. It is based upon the interaction of light with the chemical bonds within a material.

Modern confocal Raman microscopy and imaging, which allows one to obtain two- and three-dimensional spectrochemical images of samples in various states and forms, has become an indispensable research method for scientific communities, from physicists to chemists, to criminalists, to geologists, to biologists, to medics, etc.

2025年1月16日 · A visible stimulated Raman scattering microscope with extensive pulse chirping in combination with a deep-learning-based denoiser and Fourier reweighting leads to highly sensitive, label-free ...

2021年11月25日 · Raman microspectroscopy offers microbiologists a rapid and non-destructive technique to assess the chemical composition of individual live microorganisms in near real time. In...

Raman microscopes are sophisticated systems that comprise multiple components, including an optical microscope, a Raman spectrometer, a laser, and an imaging detector, such as a CCD (charged-coupled device) camera. Each component requires careful selection to configure the assembled instrument for the application at hand.