2023年1月10日 · Plasmid multimerization occurs via homologous recombination between two or more copies of the same plasmid (Bedbrook & Ausubel, 1976). This recombination event produces a multimeric form of the plasmid with repeating sequences.

2024年7月23日 · We present an MG1655 ΔrecA single-locus knockout that avoids multimer production. These results can aid synthetic biologists in improving design and reliability of plasmid-based circuits.

2003年11月6日 · Supercoiled multimeric plasmids are of particular interest for pharmaceutical purpose because they contain multiple copies of a therapeutic gene and can therefore be more efficient vectors. A process for the preparation of Escherichia coli strains replicating dimers, trimers, and tetramers of a 4.6 kb plasmid is presented.

2003年11月6日 · Multimeric plasmid molecules are of particular interest for biopharmaceutical purpose because they contain multiple copies of a functional gene and can thereby be more efficient in transfection experiments than the monomeric form. Leahy et al. (1997) produced linear plasmid multimers by in vitro enzymatic synthesis. Transfection experiments ...

2024年7月24日 · To optimize plasmid-based circuits, it is crucial to control plasmid integrity, including the formation of plasmid multimers. Multimers are tandem repeats of entire plasmids formed during replication by failed dimer resolution. Multimers can affect the behavior of synthetic circuits, especially ones that include DNA-editing enzymes.

Plasmid multimers affect plasmid stability by lowering the number of independently segregating units at the time of cell division. They may also interfere with the control of replication and with the activity of the plasmid segregation system.

2024年7月25日 · Plasmid DNA (pDNA) is an essential tool in genetic engineering that has gained prevalence in cell and gene therapies. Plasmids exist as supercoiled (SC), open circular (OC), and linear forms. Plasmid multimerization can also occur during the manufacturing process.

We report here recombination between bacterial plasmids which results in the formation of plasmid multimers. Plasmid multimers migrate as discrete bands on gels, so we can assay multimer formation by agarose gel electrophoresis of supercoiled DNA .

1976年12月1日 · We report here the formation of plasmid multimers in E. coli. Multimers are covalently closed, circular molecules composed of tandem repeats of the monomer plasmid. Multimer formation occurs at high frequency in rec+, rec B-C −, and rec F − hosts.

2012年2月14日 · We developed a general restriction enzyme-free and ligase-free method for subcloning up to three DNA fragments into any location of a plasmid. The DNA multimer generated by prolonged overlap extension PCR was directly transformed in Escherichia coli [e.g., TOP10, DH5α, JM109, and BL21(DE3)] and Bacillus subtilis for obtaining chimeric plasmids.