2020年3月2日 · For mice, if a pure, soluble protein antigen is being used and is abundant, a dose of 50-100 µg in adjuvant at each immunization is a sensible general recommendation; for rats and hamsters, a dose of 100-200 µg is sufficient. Lower doses can be used for antigens with higher immunogenicity.

Mice are injected IP or SC with the antigen/adjuvant precipitate (1-100 ug protein in 5-10% alum or other suitable adjuvant) in 100-200 mcl sterile saline. If given subcutaneously, no more than 0.05 mls will be injected per site.

Mice, rats, or hamsters are given biweekly injections of a purified antigen, cultured cells, or cDNA. Adjuvants (Freund's, Ribi, Hunter's TiterMax, ImmunEasy, or Alum) are mixed with the immunizing antigen for the first two immunizations only. Complete Freund's adjuvant is only used with the first immunization.

Mice are immunized at 2- to 3-week intervals. Test bleeds are collected 7 days after each booster immunization to monitor serum antibody levels. Mice are chosen for hybridoma fusions when a sufficient antibody titer is reached.

Immunization for hybridoma production should take into consideration route and volume. Subcutaneous and intraperitoneal routes are most commonly used. For subcutaneous immunization, no more than 0.3 ml total volume should be injected each time; no more than 0.05 ml should be injected per site.

We immunized Trianni humanized mice against two distinct targets (human PD-1 and human CTLA-4) using four different immunization protocols: ALD/MDP rapid, ISA50 rapid, ISA50 12 week, and Cells/DNA (Figure 1a).

Custom mouse antibodies—a reliable protocol for immunizing mice, collecting serum, testing bleeds, reporting titer results, and delivering antisera; Full-service integration—combine this immunization service with our complete set of services for …

For each scheduled immunization, prepare a sample consisting of a maximum of 50 micrograms of antigen in sterile PBS (or animal compatible buffer) in a volume of 50 µL per mouse. Adjuvant: 5.3.1. Use an adjuvant to increase the immunological response to poor antigens.

In applications where several different mouse MAb at high concentrations are required for injection into mice, the in vitro method can be inefficient. MAb from mouse ascitic fluids might be essential for experiments in which MAb are used in vivo in mice. d.

Mice, rats, or hamsters are immunized by giving biweekly injections of a purified antigen, cultured cells, or cDNA. For mice, if a pure, soluble protein antigen is being used and is abundant, a dose of 50–100 µg in adjuvant at each immunization is a sensible general recommendation; for rats and hamsters, a dose of 100–200 µg is sufficient.