Lambda phage is a non-contractile tailed phage, meaning during an infection event it cannot 'force' its DNA through a bacterial cell membrane. It must instead use an existing pathway to invade the host cell, having evolved the tip of its tail to interact with a specific pore to allow entry of its DNA to the hosts.

Upon adsorption on the lamb receptor of the host cell, lambda gDNA is injected through the tail which forms a hollow tube through which the DNA passes to the cell. The phage λ leads two life cycles, the lytic cycle and the lysogenic cycle after injecting its DNA into E.coli cell.

Phage lambda DNA was one of the earliest model systems for studying the physical nature of DNA and genes, and a substantial amount of early research examined the hydrodynamic properties of the lambda chromosome with the goal of, among other things, determining its absolute molecular weight.

Bacteriophage Lambda Infection and DNA Replication. A λ virion consists of a 48.5 kb dsDNA genome tightly packaged within an icosahedral protein shell and a tail, which serves to deliver the linear genome through the cell envelope into the cytoplasm of an Escherichia coli cell.

2025年1月15日 · The lambda phage genome is a linear double-stranded DNA molecule, approximately 48,502 base pairs in length. This genetic material is organized into distinct functional regions, each responsible for specific roles during the phage’s life cycle.

2024年11月23日 · The head contains the phage’s double-stranded circular DNA genome. The phage particle recognizes and binds to its host, E. coli, causing DNA in the head of the phage to be ejected through the tail into the cytoplasm of the bacterial cell.

Bacteriophage λ is a temperate phage, which, upon infection of Escherichia coli, enters either the lytic or lysogenic replication pathway. The latter is defined by the integration of the λ prophage within the bacterial host genome, where it is quiescently inherited by daughter cells.

The position and orientation of genes in lambda and lambda dg DNA are described. The position of six genes located in the right half of isolated lambda DNA was found to be - (N, i λ)-- O-P---Q-R - (right end of DNA), which is their order on the genetic map of the vegetative phage.

2015年5月1日 · Phage lambda DNA was one of the earliest model systems for studying the physical nature of DNA and genes, and a substantial amount of early research examined the hydrodynamic properties of the lambda chromosome with the goal of, among other things, determining its absolute molecular weight.

Lambda DNA is a common DNA substrate extracted from bacteriophage lambda (cI857ind 1 Sam 7) and stored in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA.