DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.

2023年8月3日 · DNA ligase plays a vital role in DNA replication, DNA repair, and DNA recombination. Now day purified DNA ligase is isolated in the laboratory, which is used in gene cloning to join DNA molecules together to form recombinant DNA.

2024年12月27日 · DNA ligase is an enzyme which helps the DNA strands become reconnected after the DNA is damaged in an environment, due to errors in replication or other stresses in the cellular environment. The ligase seals these nicks and maintains genome stability by preventing accumulation of mutations.

DNA ligase is therefore an essential enzyme required for normal DNA replication and repair in E. coli. Purified DNA ligases have proved to be useful reagents in the construction in vitro of recombinant DNA molecules.

DNA ligase is an enzyme that plays a crucial role in maintaining the structural integrity of the genome by repairing breaks in the DNA backbone. It catalyzes the formation of a bond between adjacent nucleotides in the DNA, ensuring the stability of genetic information.

DNA ligases are enzymes that catalyze the joining together of two DNA ends, in a manner that requires either adenosine triphosphate (ATP) or NAD +. DNA ligases are hugely important in molecular biology and biotechnology, particularly for their role in gene cloning.

2023年9月6日 · DNA ligase is an enzyme that catalyzes the formation of a covalent bond between a 5’ phosphate and a 3’ hydroxyl group at the termini of DNA fragments; this bond ligates the two DNA fragments together.

2024年10月19日 · DNA ligases are enzymes responsible for joining DNA strands, playing a role in maintaining genome integrity. Their ability to facilitate the formation of phosphodiester bonds is important during both DNA replication and repair processes.

DNA ligases seal 5′-PO 4 and 3′-OH polynucleotide ends via three nucleotidyl transfer steps involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are essential guardians of genomic integrity, and ligase dysfunction underlies human genetic disease syndromes.

In the remainder of this chapter, we will briefly describe the eukaryotic DNA ligases and then focus on DNA ligase I, the replicative DNA ligase, and the mechanisms that underlie the specific participation of this enzyme in DNA replication.