Getting started with loop-mediated isothermal amplification …
2022年10月12日 · LAMP takes advantage of the strand-displacing properties of Bst DNA polymerase (NEB #M0374) in conjunction with a unique primer design to allow rapid amplification of a specific sequence at one temperature. It is an ideal technique for field, point-of-care, or …
仅显示来自 neb.com 的搜索结果Loop Mediated Isothermal Amplification (LAMP) Tutorial - NEB
This LAMP dumbbell structure contains multiple sites for initiation of synthesis from the three prime ends of the open loops and annealing site…
Loop-mediated isothermal amplification - Wikipedia
LAMP is less versatile than PCR, the most well-established nucleic acid amplification technique. LAMP is useful primarily as a diagnostic or detection technique, but is not useful for cloning or many other molecular biology applications enabled by PCR. Because LAMP uses 4 (or 6) primers targeting 6 (or 8) regions within a fairly small segment of the genome, and because primer design is subject to numerous constraints, it is difficult to design primer sets for LAMP "by eye". Free, o…
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Evaluation of amplicons by AF4 as assistant for deep …
2025年1月4日 · Loop-mediated isothermal amplification (LAMP) is a rapid and efficient method for DNA amplification, producing concatemers of varying lengths (amplicons). This study explores …
Loop Mediated Isothermal Amplification (LAMP) …
This LAMP dumbbell structure contains multiple sites for initiation of synthesis from the three prime ends of the open loops and annealing sites for both the inner and loop primers. As amplification precedes from these multiple sites, the …
Loop-Mediated Isothermal Amplification - an overview
Loop-mediated isothermal amplification (LAMP) is a one-step amplification reaction that amplifies a target DNA sequence with high sensitivity and specificity under isothermal conditions (about …
Evaluation of amplicons by AF4 as assistant for deep ... - PubMed
2024年11月24日 · Loop-mediated isothermal amplification (LAMP) is a rapid and efficient method for DNA amplification, producing concatemers of varying lengths (amplicons). This study …
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Kinetics of elementary steps in loop-mediated isothermal …
2022年12月30日 · Loop-mediated isothermal amplification (LAMP) is a powerful nucleic acid amplification method that uses 4–6 primers with a strand-displacing DNA polymerase to …
Colorimetric strategies applicable for loop-mediated isothermal ...
2024年8月1日 · In recent years, loop-mediated isothermal amplification (LAMP) has gained popularity for detecting various pathogen-specific genes due to its superior sensitivity and …
Development of a Highly Multiplexed RT-LAMP Assay for …
2 天之前 · Nucleic acid amplification tests (NAATs) can achieve high accuracy for diagnosing infectious diseases by targeting conserved genetic sequences specific to the target organism. …
GitHub - jackwadden/lamprey: A Loop-Mediated Isothermal …
LAMPrey is a Loop-Mediated Isothermap Amplification (LAMP) concatemer analysis, variant calling, and polishing tool. LAMPrey follows a seed, chain, align paradigm to improve recovery …
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