In enzymology, an amidase (EC 3.5.1.4, acylamidase, acylase (misleading), amidohydrolase (ambiguous), deaminase (ambiguous), fatty acylamidase, N-acetylaminohydrolase (ambiguous)) is an enzyme that catalyzes the hydrolysis of an amide.

2020年11月1日 · Motivated by industrial demands, crystallographic investigations and catalytic mechanisms of amidases based on structural biology have witnessed a dramatic promotion in the last two decades.

Motivated by industrial demands, crystallographic investigations and catalytic mechanisms of amidases based on structural biology have witnessed a dramatic promotion in the last two decades.

2009年10月24日 · Amidase or amidohydrolase (E.C. 3.5.1.4) is an interesting member of nitrilase superfamily (Pace and Brenner 2001) which is used by prokaryotes in carbon and nitrogen fixation. In recent years, amidases turned out to be versatile biocatalyst in …

2020年11月1日 · An aliphatic amidase from P. aeruginosa was engineered by substitution of glycine at a conserved tryptophan 138 position, which allowed this enzyme to hydrolyze aromatic amides such as p-nitrophenylacetamide and phenylacetamide (Karmali et al., 2001).

Amidases of the nitrilase superfamily, which catalyze the hydrolysis of an amide, leading to the formation of carboxylic acid and ammonia, play a role in important metabolic processes.

Amidase as a versatile tool in amide-bond cleavage: From molecular features to biotechnological applications. Zheming Wu, ... Yuguo Zheng, in Biotechnology Advances, 2020. 4.2 Catalytic mechanism of amidase. In general, amidases catalyze …

Amidases are amide bond-cleaving enzymes that have been extensively investigated during the last 20 years. They catalyse the hydrolysis of amides to carboxylates and ammonia and exist in all kingdoms of the living world. Most of the currently known amidases have been found and described in bacteria.