S-Adenosyl methionine (SAM), also known under the commercial names of SAMe, SAM-e, or AdoMet, is a common cosubstrate involved in methyl group transfers, transsulfuration, and aminopropylation. Although these anabolic reactions occur throughout the body, most SAM is produced and consumed in the liver. [ 1 ]

2024年12月1日 · Using the synergistic activation mediator (SAM) system, we unexpectedly find that regardless of the targeted sequence any sgRNA induces global genome-wide DNA methylation. We term this method...

DNA甲基化(methylation)是一种表观遗传修饰,它是由 DNA甲基转移酶 (DNA methyl-transferase, DNMT)催化 S-腺苷甲硫氨酸 (S-adenosylmethionine, SAM)作为甲基供体,将DNA的CG两个 核苷酸 的胞嘧啶被选择性地添加甲基,主要形成 5-甲基胞嘧啶 (5-mC)(常见于基因的5'-CG-3'序列)和少量 …

SAM is the sole methyl group donor involved in the methylation of DNA, RNA and histones, modulating the autophagic process by mediating epigenetic effects. Moreover, the metabolites of SAM, such as homocysteine, glutathione, decarboxylated SAM and spermidine, also exert important influences on the regulation of autophagy.

S-Adenosylhomocysteine (AdoHcy), formed after donation of the methyl group of AdoMet to a methyl acceptor, is then hydrolyzed to adenosine and homocysteine by AdoHcy hydrolase. This enzyme has long been a target for inhibition as its blockade can affect methylation of phospholipids, proteins, DNA, RNA, and other small molecules.

SAM can alter DNA methylation, possibly through altering the SAM/SAH ratio in the cell which leads to inhibition of DNA methyltransferase activity . Indeed, our data show that SAM treatment causes widespread changes in DNA methylation across many chromosomes (Figure 3). Notably however, the changes in DNA methylation occur in both directions ...

2012年12月21日 · S-adenosyl methionine (SAM) is a universal biological cofactor that is found in all branches of life where it plays a critical role in the transfer of methyl groups to various biomolecules, including DNA, proteins and small-molecule secondary metabolites. The methylation process thus has important i …

2021年7月1日 · As the second most ubiquitous cofactor in humans, S-adenosyl- l -methionine (SAM or AdoMet) serves as a methyl donor for SAM-dependent methyltransferases (MTases), which transfer a methyl group to a nucleophilic acceptor such as …

Like radical SAM methyltransferases, MTTases require two molecules of SAM per turnover, one for dAdo radical production and one to provide the methyl group. The observed products from these two uses of SAM are methionine and dAdo from SAM cleavage and SAH from methylation.

2024年12月1日 · Using the synergistic activation mediator (SAM) system, we unexpectedly find that regardless of the targeted sequence any sgRNA induces global genome-wide DNA methylation. We term this method SAM-DNMT3A and show that induction of global DNA methylation is a unique vulnerability in ER-positive breast cancer suggesting a …