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质粒载体构建全解密:从基础到应用的实验指南
而在我们进行质粒构建的过程中,整个步骤可以简单理解为七个环节。 第一步是获取目的片段。这是质粒构建的基础,也是后续实验的出发点。以构建过表达载体为例,我们通常需要选择目标基因的cds区。例如,想要构建小鼠P65基因,我们可以在NCBI网站上获取 ...
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