It has visible laser lines at 458nm, 488nm, 514nm, 561nm and 633nm. The Leica SP8 is also a multi-photon microscope that can utilize the internal detectors from the visible confocal as well as two ...

In the last decade, two techniques have revolutionized fluorescence microscopy: the development of genetically encoded fluorophores, such as GFP 5,6 and the invention of two-photon microscopy 7.

By imaging much faster and with less harm to brain tissue than traditional two-photon microscopy, the new approach could provide a clearer view of how neurons communicate in real time, leading to ...

Two-photon polymerization is a 3D nanoscale manufacturing ... Furthermore, conductive atomic force microscopy studies have shown that the full metallization of the structure surface is realized.

In situ diagnosis Multiphoton microscopy combines second- and third-harmonic generation (SHG and THG), along with two- and three-photon excitation, to rapidly visualize molecular and structural ...