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生物通
9 天
软壳龟线粒体基因组研究:解开进化谜题,揭示物种奥秘
研究人员采用了多种关键技术方法。在样本采集方面,分别从广西桂平的养殖场和湖南永州野外捕获了 Palea steindachneri 和 Pelodiscus axenaria 样本。之后,利用 MicroElute Genomic DNA Kit 提取基因组 DNA。对于 P. steindachneri,通过设计 11 对引物进行 PCR 扩增、克隆和测序来 ...
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