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Comparison of “framework Shuffling” and “CDR Grafting” in humanization of a PD-1 ...
E.coli DH10B was a product of Invitrogen(Carlsbad, CA, USA). E.coli XL-1Blue was obtained from Agilent Technologies(USA). M13KO7 helper phage was obtained from NEB(Beijing, China). PrimeSTAR ® HS DNA ...