This useful study presents findings on the developmental roles of Nup107, a key nucleoporin, in regulating the larval-to-pupal transition in Drosophila melanogaster through its involvement in ecdysone ...

Basic Science The esterase method of detecting leukocytes in a urine sample relies on the fact that esterases are released from lysed urine granulocytes (neutrophils). In the dipstick method ...

Gene expression studies by quantitative reverse-transcription PCR (qRT-PCR) can be challenging when attempted with samples of a few cells or a single cell. Epicentre's Message BOOSTER ™ cDNA ...

These useful findings assigned a novel functional implication of histone acylation, crotonylation. Although the mechanistic insights have been provided in great detail regarding the role of the YEATS2 ...

For use with our PCR rapid detection systems, BIOTECON Diagnostics has developed fast and easy-to-use DNA extraction kits. The foodproof ® ShortPrep, StarPrep, Sample Preparation and Magnetic ...

Antibodies were incubated with Dynabeads protein G (Invitrogen, Carlsbad, US) at 4°C for at least 30 minutes. Cells were lysed in NP-40 buffer (Beyotime, Shanghai, China) supplemented with protease ...

Then, cells were harvested for further study. Cells were harvested from cultured dishes and were lysed in a lysis buffer [20 mM Tris-HCl pH 7.6, 1 mM EDTA, 140 mM NaCl, 1% NP-40, 1% aprotinin, 1 mM ...

LV thrombi were successfully lysed in 10 of 16 patients. None of the patients suffered from clinical embolism, and therapy had to be discontinued in only one patient due to haematuria. w33 In a later ...