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Cas12活性的可调控制促进了通用和快速的一锅核酸检测
当 crRNA 与 S - free - heparin 竞争时,未观察到这种现象(图 2j ... 与不含肝素钠的系统相比,检测灵敏度提高了 10000 倍以上(图 3c,f 和补充图 11b,d)。 研究人员进一步分析了 RPA - Cas12a 系统中靶核酸的扩增情况。为评估不同条件下的扩增曲线,研究人员分别 ...
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