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FITC-D-Fructo-NHS:糖代谢研究的活化标记探针
FITC-NHS在pH 8.5条件下与D-果糖的羟基反应,标记度通过紫外分光光度法定量(1.8mol FITC/mol Fructose)。 体外实验显示,FITC-D-Fructo-NHS与GLUT5转运体的结合常数(Kd=1.3μM)与未标记品无显著差异。 将FITC-D-Fructo-NHS静脉注射至果糖不耐受模型小鼠,通过荧光成像实时监测 ...
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