Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi’an 710127, People’s Republic of China State ...
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Transfer the supernatant diluted 4 times to the ELISA plate. Then, the conjugate reagent were added to samples mixture and incubated for an hour under 37°C. And the samples were successively treated ...
Cells were separately transfected with siRNA at a final concentration of 1 μM or 0.5 μg plasmid by using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s ...