and samples were sonicated using sonication beads (Diagenode; Cat #C01020031) and the Bioruptor Pico Sonicator (Diagenode; Cat #B01060010) for 20 cycles (each cycle 30 seconds on/30 seconds off) or ...

These modifications increase chromatin accessibility, influencing growth, differentiation, and ion transport, with contrasting effects on normal and colorectal cancer cells. Study: Short-chain ...

Here, we demonstrate that inhibition of Aurora kinase A (AurA) dampens trained immunity induced by β-glucan. ATAC-seq and RNA-seq analysis reveals that AurA inhibition restricts chromatin ...

Accordingly, we have a limited understanding of how exactly p65/RELA cooperates with partner TFs (in cis at overlapping DNA elements or in trans across chromosomes), chromatin modifiers, the ...

Genomic DNA is assembled into chromatin by histones, and extruded into loops by cohesin. These mechanisms control important genomic functions, but whether histones and cohesin cooperate in genome ...

The chromatin landscape is a key aspect of epigenetic regulation in eukaryotes. Old histones are recycled during DNA replication, therefore providing a blueprint for the duplication of their ...

ChIPs were performed with 3-5x10 6 cells as previously described (Rodríguez-Ubreva and Ballestar, 2014). Cells were crosslinked with 1% methanol free-formaldehyde (PFA) and chromatin was sheared by ...