Chromatin immunoprecipitation (ChIP ... to DNA-binding proteins with formaldehyde and mechanically sheared using sonication. The DNA-protein complex is then precipitated with an antibody that ...

These modifications increase chromatin accessibility, influencing growth, differentiation, and ion transport, with contrasting effects on normal and colorectal cancer cells. Study: Short-chain ...

Gene-editing strategies involving engineered nucleases [i.e., zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), meganucleases, and clustered regularly interspaced ...

DNA was fragmented by sonication and sequenced. We carried out MNase-Seq as previously described ... and incubated at room temperature for 20 mins. Chromatin digested with 1.9 U– 7.5 U micrococcal ...

This valuable study is a detailed investigation of how chromatin structure influences replication origin function in yeast ribosomal DNA, with a focus on the role of the histone deacetylase Sir2 and ...

After homogenizing the sample in cell lysis buffer the nucleus phase was pelleted and then suspended in nucleus lysis buffer. Samples were then sheared by sonication (Bioruptor UCD-300, Diagenode) and ...

Chromosome segregation relies on kinetochores that assemble on specialized centromeric chromatin containing a histone H3 variant. In budding yeast, a single centromeric nucleosome containing Cse4 ...

Program in Chemical Biology, Department of Chemical Physiology and Biochemistry, Oregon Health & Science University, 3181 SW Sam Jackson Park Rd, Portland, Oregon 97239, United States ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. To directly examine the interplay between ...