Running these different assays in parallel is “hugely attractive”, Kampmann says. The CRISPR-activation screens in particular “are really very powerful as a supplement to loss-of-function ...

These regulated pathways must be studied in depth in order to avoid selective pressure favoring anti-CRISPR systems activation, hence prevalence of resistance in a more aggressive manner." ...

undesired gene activation or inactivation, cytotoxicity, and immune responses. Off-target genome editing could significantly reduce the therapeutic potential of CRISPR-Cas9. 1 Furthermore ...