The assay mixture contained 50 mM Tris pH 7.8, 2.5 mM MgCl 2, 1 mM ATP, 5 mM DTT, 0.15 U phosphofructokinase (from Bacillus stearothermophilus from Sigma Aldrich catalog number F0137), varying ...

Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China ...

Table 1. Comparison of the 5AVA production by different engineered microbial strains and production of amino acids by B. methanolicus. Even though the application of diverse 5AVA biosynthetic pathways ...