iVar is a computational package that contains functions broadly useful for viral amplicon-based sequencing.

As SARS-CoV-2 variants of concern emerged, the genome sequencing of SARS-CoV-2 strains became more important. In this study, SARS-CoV-2 was sequenced using amplicon-based genome sequencing with MinION ...

2016), and Genotyping-in-Thousands by sequencing (GT-seq) (Campbell et al., 2015), have been reported. Here, we performed a comparison between these two methods and MTA-seq with respect to factors ...

Pyrosequencing has revolutionized microbial community analysis by allowing the simultaneous assessment of hundreds of microbial communities in multiplex with sufficient depth to resolve meaningful ...

R package for DNA methylation analysis on Direct Whole Methylome Sequencing (dWMS) data.

A new study led by Prof. Tan Zhiliang from the Institute of Subtropical Agriculture of the Chinese Academy of Sciences has ...

The advent of next-generation sequencing has made possible genome ... platform-specific adaptor ligation. This process typically results in considerable sample loss with limited throughput.

DNA sequencing is the process used for determining the order of the nucleotide bases—adenine, guanine, cytosine, and thymine—in a molecule of DNA. DNA sequencers are scientific instruments ...

Monoallelic loss of PTEN and ARID1A is identified in normal colonic epithelium. The resulting haploinsufficient phenotypes ...

[8–12] A less known hybridization-based enrichment strategy uses filters, marked with amplicons ... hereby completing the capture process. Afterward, the enriched DNA library is eluted from ...

This valuable work presents how PRDM16 plays a critical role during colloid plexus development, through regulating BMP signaling. Solid evidence supports the context-dependent gene regulatory ...