Pyrosequencing analyzes single-stranded DNA templates by synthesizing complementary strands. Briefly, the four nucleotides (A, T, G and C) are added sequentially by a Pyrosequencing instrument to ...

Pyrosequencing has revolutionized microbial community analysis by allowing the simultaneous assessment of hundreds of microbial communities in multiplex with sufficient depth to resolve meaningful ...

San Diego, CA, USA). First, bisulfite modification was performed with 500 ng of total DNA using an EZ DNA methylation kit (Zymo Research). Modified DNA was amplified using pyrosequencing primers ...

EpigenDx's capacity and experience make us the perfect partner during pre-clinical research and development stages of the diagnostic method development. Our services consist of Targeted NGS ...

2 Ipatimup – Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal 3 Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal ...

The Hashimoto thyroiditis (HT) is an organ-specific autoimmune disease and one of the most common diseases worldwide (1). HT is characterized by chronic inflammation, with tertiary lymphoid follicles ...

Objective An adequate bowel cleansing is essential for a successful colonoscopy. Although purgative consumption is safe for the patient, there is little consensus on how the intestinal microbiota is ...