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A total of 80 mL of whole blood was used to isolate peripheral blood mononuclear cells (PBMCs), as previously described (50, 63). PBMCs count and cell viability were determined using trypan blue stain ...

We further found that neurons with accumulation of phospho-tau immunostaining contained rG4s, that rG4 structure can drive tau aggregation, and that rG4 staining density depended on APOE genotype ...