This is an important and solid study that examines the role of TFAM, a protein that helps maintain mtDNA, in mtDNA mutator mice. The authors have demonstrated that TFAM's counteractive role in mtDNA ...

Cells were quickly cooled by adding 5× volume of ice-cold PBS, lysed with 0.2% NP-40, Tris-HCl (20 mM, pH 7.4), NaCl (150 mM), sodium vanadate (2 mM), 10% glycerol and the protease inhibitor ...