1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...

Download this application note from Thermo Fisher Scientific to discover an approach using the Neon NxT system that researchers can follow for optimal transfection results. Lab safety rules dictate ...

But as with other transfection methods, the optimal conditions for electroporation of untested cell lines must be determined experimentally. 1| Collect the cells to be transfected from cultures in ...