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结核菌素和确定抗原鸡尾酒在自然环境中用于检测bcg疫苗接种牛的牛 ...
使用 Ziehl - Neelsen(ZN)染色技术确认培养阳性菌落中是否存在抗酸杆菌(AFB)。 (十)DNA 提取与 PCR 检测 使用 DNeasy Blood and Tissue Kit(QIAGEN)从培养分离物中提取 DNA,并进行了一些修改。提取的 DNA 样本在 AHI 进行两步实时多重 PCR 扩增。第一步,使用针对 IS1081 ...
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