NS0-derived mouse CLEC4F/CLECSF13 protein, with an N-terminal 9-His tag was ordered from R&D Systems which the soluble extracellular domain Ala65-Gly548 of Clec4F was ... exclusion chromatography on ...
The PME activity was determined according to Yang et al. (2019) with some modifications. Briefly, tea leaf samples were ground in ice with 5 mM phosphate buffer containing 1 M NaCl and 5% PVP (pH 7.5) ...
The in vitro γ-secretase assay was conducted as previously detailed 12. In summary, 30 nM of either wild-type or FAD mutant γ-secretase was preincubated for 30 minutes at 37 ℃ in an assay buffer ...
Institut für Chemie und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany ...
Department of Chemical and Pharmaceutical Sciences, INSTM UdR Trieste, University of Trieste, Via Licio Giorgieri 1, 34127 Trieste, Italy ...
The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana.