2.使用过程中务必保持产品干燥，避免阳光直射。 1.Mitotracker Orange：这是一种荧光染料，能够特异性地结合到线粒体上。它的荧光特性使其在显微镜下易于观察，通常呈现橙色或红色荧光。 2.CM-H2TMRos：这是一种活性氧敏感基团，能够与线粒体内的活性氧（如超 ...

Mitotracker Orange CM-H2TMRos 通常以DMSO溶解，使用时稀释至培养基中，孵育细胞后进行显微镜观察。 染色后的细胞可进行固定处理，荧光信号在固定后仍能稳定保留。 反应机理: Mitotracker Orange CM-H2TMRos是一种阳离子型橙色荧光染料，用于活细胞中线粒体的特异性染色。

ATP, betulinic acid, and staurosporine were purchased from Sigma-Aldrich (St. Louis, MO). MitoTracker Deep Red FM was purchased from Thermo Fisher Scientific (Massachusetts, United States). Antibodies ...

此前研究已表明，补体体在造血干细胞 / 祖细胞（HSPCs）和骨髓基质细胞中表达，对调节它们的运输、增殖等过程至关重要。然而，补体体缺陷小鼠在造血方面存在诸多问题，比如 C3 基因敲除（C3-KO）小鼠和 C5 基因敲除（C5-KO）小鼠对氧化应激的反应不同，这背后的代谢机制却并不清楚。为了深入探究这些问题，来自华沙医科大学临床前研究与技术中心、路易维尔大学干细胞研究所的 Adrian ...

Scientists at the University of Buffalo have made a breakthrough in the quest to slow or even reverse aging. Their research ...

XT_PGX_MmV1 Immunology kit was used following manufacturer’s instructions. MitoTracker Green FM (1/1500) and MitoTracker Deep Red (1/1000) were added in FACS buffer for 15 min at RT (ThermoFisher ...

Revised: This Reviewed Preprint has been revised by the authors in response to the previous round of peer review; the eLife assessment and the public reviews have been updated where necessary by the ...