In these experiments, low-passage HEK 293T, HeLa, MCF7, HCT116, K562, and JLat cells were plated in SensoPlate 24 Well F-Bottom ... HO’G assisted in conception and design of experiments. NBH created ...

CRISPR-Cas systems are prokaryotic immune systems that confer resistance to foreign genetic elements such as plasmids and phages. CRISPR-Cas systems have been exploited for targeted genome editing ...

Shares of CRISPR Therapeutics CRSP have lost more than 30% in the past year. Though the stock soared at the onset of 2024, thanks to the FDA approval of its one-shot gene therapy Casgevy for two ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Gene editing stands for the methods to precisely make changes to a specific nucleic acid sequence. With the recent development of the clustered regularly interspaced short palindromic repeats (CRISPR) ...

C-terminal amidation is a PTM that involves the addition of an amid group to the C-terminus of a peptide or protein. Currently there is no direct evidence to suggest that C-terminal amidation of ...

近年来，研究人员发现了一种新的蛋白质降解信号——C末端酰胺（C-terminal ...

CRISPR 干扰（CRISPRi）技术是研究基因功能的有力工具 ... 因此，开发一种能够扩大靶向范围、提高筛选效率的 CRISPRi 技术至关重要。 研究选用了 Jurkat、K562、MV4-11、HEK293FT 和 A375 等多种细胞系。其中，Jurkat、K562 和 MV4-11 细胞在 RPMI 培养基（Gibco）中培养，添加 ...

1Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5900 USA 2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical ...