This protocol was adapted from “Stage 1: Synthesis of first strand cDNA catalyzed by reverse transcriptase” and “Stage 2: Second strand synthesis,” in Molecular Cloning: A Laboratory ...

Total RNA was extracted from gonads which were homogenized in 1 mL TRIZol (Invitrogen, USA) by shaking (60 HZ for 1 min ... equivalent amounts of the total RNA (1000 ng) were used for cDNA synthesis ...

We then purified and stored the TRNA as described above for the cell culture samples. We followed the manufacturer’s instructions from the SuperScript IV First-Strand Synthesis System (cat# 18091050, ...

We've tested hundreds of smart home products in more than 20 categories to help determine which ones are best for every room in (and out of) the house. I'm PCMag's managing editor for consumer ...

HORRY COUNTY, S.C. (WBTW) — Horry County Schools Superintendent Clifford Jones issued a brief message to parents Sunday night ahead of his first day on the job, promising to continue investments ...

This lineage is traced through Prophet’s cousin and son-in-law, Ali, the first Imam, and his wife, Fatima, Prophet’s daughter. Aga Khan IV inherited the title of the 49th Imam of the Shia Imami ...

The Bank of South Sudan (BoSS) is set to launch the country’s first National Instant Payment System (NIPS) in collaboration with the AfricaNenda Foundation. This announcement was contained in a ...

Reliable communication tools are therefore essential. The basic idea of the WWW was to merge the evolving technologies of computers, data networks and hypertext into a powerful and easy to use global ...