A total of 84 explants were evaluated. From a prescreening assay with 13 ISSR primers, six markers were chosen because they generated polymorphic, reproducible, well-differentiated, and bright bands.

Eight ISSR primers were used for primer selection; UBC 808, UBC 809, UBC 811, UBC 830, UBC 855, UBC 880, UBC 888, and UBC 891. These primers were based on the methodology described by Ismail et al.

PCR-based techniques are methods that rely on the polymerase chain reaction (PCR) to amplify stretches of DNA by creating many identical or near-identical copies. For example, PCR amplification ...

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