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YY1 介导的 DUXAP8 通过调控 DEPDC1 表达促进肝癌进展:潜在的治疗新靶点
接着通过细胞培养技术培养了 Huh7、Hep3B 和 THLE - 3 等细胞系。在检测基因和蛋白表达方面,使用了定量逆转录聚合酶链反应(qRT - PCR)和蛋白质免疫印迹法(Western blot)。此外,还运用了荧光素酶报告基因实验(Luciferase reporter assay)、RNA 免疫沉淀实验(RNA ...
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