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生物通
17 天
噬菌体感染时,顺序出现的膜结合和蛋白结合细胞器对基因组的区室 ...
实验设计 观察感染早期囊泡形成:通过冷冻电镜断层扫描(cryo-ET)观察野生型(WT)Goslar 感染大肠杆菌(E. coli)后 1 分钟(mpi)的细胞,确定囊泡是否在感染早期形成;利用细胞可渗透的膜染色剂 MitoTracker Green FM(MTG)和 DNA 染色剂 DAPI 进行荧光显微镜观察 ...
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