After incubation, the extracts were aspirated, and cells were washed thrice with phosphate buffer saline. Each well was incubated with the mixture of calcein AM and ethidium homodimer III (2 μL ...

Live cell imaging solution (1X), ethidium homodimer-1 (EthD-1), calcein AM and Hoechst 33342, trihydrochloride and trihydrate, and 12 and 96 well plates were purchased from Invitrogen by Thermo Fisher ...

As previously described (40), a tissue section (0.5 mm thickness) was cut out of the unfixed explants and incubated for 40 min in a staining solution (1 µM calcein, 2 µM ethidium homodimer-1).

Creative Commons (CC): This is a Creative Commons license. Attribution (BY): Credit must be given to the creator. Non-Commercial (NC): Only non-commercial uses of the work are permitted. Tissue ...

Aim: Published clinical series suggest the osteoodontokeratoprosthesis (OOKP) may have a lower extrusion rate than current synthetic keratoprostheses. The OOKP is anchored in the eye wall by ...