RRBS and targeted bisulfite sequencing (MethylTarget) were performed by Genesky Biotechnologies Inc. (Shanghai, China). A detailed description of the RRBS and MethylTarget assay was described in our ...

Single-cell DNA methylation sequencing technology has brought new perspectives to investigate epigenetic heterogeneity, supporting a need for computational methods to cluster cells based on ...

Researchers at the University of Toronto have invented a new method that uses DNA sequencing to measure metabolites. This enables rapid and precise analysis of biological compounds, such as sugars ...

Mapping DNA methylomes in single cells is challenging, and thus studies using bulk samples remain common. Here, authors develop a transformer-based method for methylation pattern analysis to ...

A new method, "smol-seq," utilizes DNA sequencing for rapid and precise metabolite detection. It employs DNA aptamers, each with a unique barcode, to bind specific metabolites.

Unlike traditional methods, this technique does not require de novo DNA synthesis (assembling the molecule's components ... d) Parallel printing via DNMT1-guided catalysis to selectively write ...

One potential explanation lies in epigenetics —the study of how environmental factors can influence gene activity without altering the DNA sequence ... method, we identified groups of DNA ...

The methylation of plasma cell-free DNA (cfDNA) has emerged as a valuable diagnostic and prognostic biomarker in various cancers including colorectal cancer (CRC). Currently, there are no biomarkers ...

The SPRQ chemistry brings three major benefits: 4x lower DNA input, 33% higher sequencing yield and more accurate direct methylation detection. With only 500 ng of DNA needed for a human whole genome, ...