Running these different assays in parallel is “hugely attractive”, Kampmann says. The CRISPR-activation screens in particular “are really very powerful as a supplement to loss-of-function ...

These regulated pathways must be studied in depth in order to avoid selective pressure favoring anti-CRISPR systems activation, hence prevalence of resistance in a more aggressive manner." ...

The second tool is the CRISPR-Cas9 system, which allows easy and precise editing of any region of the genome. When it comes to traditional immortalized cell lines, such as HeLa or HEK293, cutting with ...