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实验室安全警报:解码四大高危试剂的隐形杀戮链与生存法则
科研人员在接触DEPC时往往忽略其致癌风险,这种RNA酶抑制剂通过与组蛋白发生氨基甲酰化反应,其代谢产物N-硝基化合物会直接损伤DNA修复机制。美国国立卫生研究院毒理学数据库显示,DEPC的半数致死量(LD50)仅为540mg/kg,相当于成年人体重60kg摄入32.4克即达危险阈值。而实验室常用的0.1%DEPC水溶液处理环节中,操作者实际暴露量常超出安全标准3-5倍。
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