ProbeLibrary, a fast, specific and flexible format for quantitative real-time PCR, is described. The ProbeLibrary concept is based on the fact that just 90 short probes provide transcriptome-wide ...

Locked nucleic acid (LNA) probes Probes that use modified nucleotides with a locked ribose ring, increasing the thermal stability of the probe-target hybrid While their specificity and ... Some ...

But detection with dyes like SYBR Green I is less specific than probe-based detection methods. For example, if primers bind to each other, the dye will bind to these so-called primer dimers and ...

The ubiquity of quantitative PCR (qPCR) for interrogating gene expression in a variety of research and clinical contexts makes it an indispensable laboratory tool. Among the most critical variables ...

The GoTaq ® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and ...

Scorpions ™ Primers integrate primer and probe into a single bifunctional molecule. Unlike dual-labeled probes and Molecular Beacons, Scorpions Primers combine primer and probe in one molecule ...