1| To synthesize first-strand cDNA, mix the following in a sterile microcentrifuge tube on ice ... Mix the reagents by gently vortexing and centrifuge the reaction mixture briefly in a ...
1| In a sterile 0.5-ml microcentrifuge tube or amplification tube (on ice), mix the following reagents for the first amplification reaction: 2| Place the tubes in a thermocycler and amplify the ...
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