Well-spread meiotic pachytene bivalents were obtained by using the prolonged hypotonic treatment combined with high chloroform Carnory' fixative solution from cells of the testes of domestic pigs.

Together with the reduction of quadrivalent frequency, a complementary increase in the number of bivalents (from 8.63 to 11.01) was noticed. A few uni- and tri-valents were also present at ...