but little is known about the fidelity of transcriptional initiation by RNA polymerase II in vivo. I suggest that ∼90% of Pol II initiation events in yeast represent transcriptional noise ...

In yeast, an important part of pervasive transcription ... It allows the mapping of elongating RNA polymerase II 27. In this technique, cDNA probes are hybridized to DNA microarrays that carry ...

There are a variety of different types of RNA polymerases that can transcribe different types of RNA. RNA polymerase II, shown here, is responsible for creating messenger RNA (mRNA), which is the main ...

The N-terminal ATP-dependent ligase domain belongs to the covalent nucleotidyltransferase enzyme superfamily that includes classic RNA/DNA ligases and mRNA capping enzymes. We’ve shown that: (i) each ...

In the model system I study, Saccharomyces cerevisiae (Baker’s yeast), the RNA polymerase enzyme is aided by many proteins known as elongation factors. These factors may be involved in dealing with ...

They were analyzing two primary components of the transcription system of RNA polymerase II multisubunit enzyme—carboxy-terminal domain (an IDR) and Ess1 prolyl isomerase—in five cold- and ...