The cost of making a Phycoerythrin conjugated tetramer is $800. The most expensive reagents are the biotin ligase (BirA from Avidity, LLC) and the PE-conjugated streptavidin (Prozyme). On occasion, it ...

The choice of label is based upon the signal-to-noise ratio of the label, the capabilities of the flow cytometer that will be used for the assay, and the availability of other fluorescent antibodies ...

All stainings are carried out at 4°C. Sometimes, higher intensity tetramer stains are obtained if incubated at room temperature or above, but some surface markers — particularly CD62L— are sensitive ...